The Equilibrium Unfolding Parameters of Horse and Sperm Whale Myoglobin
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چکیده
منابع مشابه
The Eguilibrium Unfolding Parameters of Horse and Sperm Whale Myoglobin
The urea and guanidine hydrochloride (GuHCI) unfolding of sperm whale ferrimyoglobin and of horse ferrimyoglobin has been studied under equilibrium conditions at both neutral and acid pH. Using two spectral parameters (the Soret absorbance and the molar ellipticity at 222 nm) to monitor conformational equilibria, and various thermodynamic treatments of the resulting data, it was found that the ...
متن کاملAcid and alkaline forms of the higher oxidation state of kangaroo, horse, and sperm whale myoglobin.
Myoglobin(IV), the derivative of myoglobin at the formal oxidation state IV, prepared from kangaroo (Megaleia rufa), horse, or sperm whale myoglobin, when cooled to liquid nitrogen temperature, assumes acid and alkaline forms with different optical spectra. The essential features of the optical spectra of the acid forms are the same as those of leghemoglobin(IV) and are very similar to those of...
متن کاملElectrostatic Interactions in Sperm Whale Myoglobin
The electrostatic free energy contribution to the stability of sperm whale ferrimyoglobin was evaluated according to the static accessibility modified TanfordKirkwood model. The electrostatic free energy contribution of each distinct structural element was divided into one term arising from interactions between it and other elements (interelemental) and another from interactions within the part...
متن کاملThe Crystal-Solution Problem of Sperm Whale Myoglobin
The central question to be discussed in this paper is whether the structure established for sperm whale myoglobin in the crystalline state is the same as that of the protein in solution. As judged by its ultraviolet optical rotatory dispersion, the helical content of metmyoglobin in solution does not differ from that in the crystal, 77 per cent. Although an uncertainty of about +/-5 per cent mu...
متن کاملSemisynthesis of sperm whale myoglobin by fragment condensation.
Reconstruction of the sperm whale myoglobin structure was accomplished by a series of aqueous condensations of suitably protected synthetic myoglobin fragments to a large fragment prepared from the native protein. Reaction of N alpha,N epsilon 19-acetimidomyoglobin with 3-bromo-2-(2-nitrophenylsulfenyl)skatole (BNPS-skatole) yielded the fragment corresponding to residues 15-153. The covalent st...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43709-5